ansR [2017-8-11 16:0:3]

transcriptional repressor (Xre family) of the ansA-ansB operon

locus: BSU23590

pI: 4.81

mw: 13.09 kDa

protein length: 116 aa

gene length: 348 bp

function: negative regulation of the ansA-ansB operon

product: transcriptional regulator (Xre family)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.6|Utilization of asparagine/ aspartate]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 2,456,990 → 2,457,340

Phenotypes of a mutant

• the ''ansR'' mutation allows glutamate utilization of a ''[gene|56CBEBCFEF5CFB4A0175498338C7AF2F45EAA3E3|rocG] [gene|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|gudB]'' mutant [Pubmed|21219666]

The protein

Protein family

• [SW|Xre family]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]
  description: [Pubmed|8478318]

  regulatory mechanism

  • [protein|146947C974D62BCB282BA3F5B924B35695D75886|AnsR]: negative autoregulation, undefined, in [regulon|146947C974D62BCB282BA3F5B924B35695D75886|AnsR regulon]

  regulation

  • induced in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]

Biological materials

Mutant

• GP1152 (del tet) available in the [SW|Stülke] lab

Expression vector

• expression of native ''ansR'' in ''B. subtilis'': pGP873 (in [SW|pBQ200]), available in [SW|Stülke] lab

References

• 8478318,11914346,21219666